miRNA Expression Profiling -
Overview
Total RNA or low molecular weight-enriched RNA is converted into cDNA using a single "megaplex" reverse transcriptase reaction.
The megaplex reaction contains a specific stem-loop primer for each of the mature target microRNA's (Each stem-loop primer is designed to hybridize
to only the fully mature microRNA, and not to precursor forms of its target.). The cDNA is subjected to a "pre-amplification" PCR.
The diluted pre-amplification products serve as substrates for TaqMan® quantitative PCR reactions. The
assays that comprise this process
are manufactured by Applied Biosystems.
TLDA cards - A profiling service is available on each of the following TaqMan Low Density Array (TLDA) cards:
For the version 2 cards, a sample may be submitted for processing on either the A card, or the B card, or both the A and B cards.
Note: In the past, the rodent panel was listed as ver 2.0, but we have changed the name to 1.0 to avoid confusion with
ABI's newest rodent card, which is also called 2.0
For support purposes, here are the layouts of the obsolete human open-plate miRNA panel
and Human miRNA ver 1.0 panel with assay names updated to Sanger 11.0
Next: Pricing
|
|
|
